Enrichment Of Committed, Chondroitin Sulfate-expressing Human Nucleus Pulposus Cells Over Progenitors Under Alginate Encapsulation
نویسندگان
چکیده
Yi Sun, Minmin Lv, Zhaomin Zheng, Danny Chan, Kenneth Cheung, Victor Leung. Dept. Biomedical Engineering, Rensselayer Polytechnic Institute, Troy, NY, USA, Dept. Orthopaedics and Traumatology, The University of Hong Kong, Hong Kong SAR, Hong Kong, Advanced Technology Research Institution of China Science Institution, Shenzhen, China, Department of Spine Surgery, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China, Dept. Biochemistry, The University of Hong Kong, Hong Kong SAR, Hong Kong.
منابع مشابه
TGF-β Induces Up-Regulation of Chondroitin Sulfate Synthase 1 (CHSY1) in Nucleus Pulposus Cells Through MAPK Signaling.
BACKGROUND/AIMS Chondroitin sulfate synthase 1 (CHSY1) is a glycosyltransferases involved in the biosynthesis of chondroitin and dermatan sulfate glycosaminoglycan (GAG). TGF-β can stimulate sulfated GAG production in nucleus pulposus cells; however, the underlying mechanisms are poorly understood. METHODS CHSY1 expression was examined in rat nucleus pulposus treated with TGF-β using real-tim...
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Intervertebral disc degeneration (IDD) is characterized by dehydration and loss of extracellular matrixes in the nucleus pulposus region. Chondroitin sulfate has been found to be the water-binding molecule that played a key role in IDD. Although investigators have reported that inflammatory cytokines are involved in the reduction of chondroitin sulfate in IDD, but the underlying mechanism is un...
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STUDY DESIGN The mRNA levels of aggrecan and collagen were quantified in intervertebral disc cells cultured under three conditions: primary alginate culture, monolayer culture, and re-encapsulation in alginate after monolayer culture. OBJECTIVES To compare the phenotype of intervertebral disc cells under different culture conditions and to investigate the reversibility of cell phenotype after...
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Intervertebral disks (IVD) acts as shock absorber between each of the vertebrae in the spinal column by keeping the vertebrae separated when the shock caused by the action. They also serve to protect the nerves that run down the middle of the spine and intervertebral disks. The disks are made of fibrocartilaginous material. The outside of the disk is made of a strong material called the annulus...
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